Ice cores were taken with a 9 cm inner diameter Kovacs ice corer. Ice cores were melted at 4 deg C with added filtered seawater (100 ml per 1 cm core section). Total volume of the melted sample was measured, the sample gently mixed, and 100 ml filled in brown glass bottles and preserved with 1 ml GAA Lugol for protist analysis. The remaining volume was sieved over 64 um (note: 20 um sieve requested in protocol was not available), rinsed into vials with a squeeze bottle and preserved in ~2-4% Zoofix for meiofauna analysis. Protocol 15.5.7 from Sampling protocol version 1 was used, except that sections 0-1 and 1-3 cm were combined into one since 0-1 cm was impossible to saw in the melting conditions we experienced.
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