P7 (NLEG25/NPAL16)

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 72f9be4e-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: 8e89c662-8f12-5fe1-ac05-e110056f3309
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 5
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 60.16
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: After 24 hour incubation

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 6f6fa31a-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: 8e89c662-8f12-5fe1-ac05-e110056f3309
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 5
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 60.16
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 70c933ac-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: 8e89c662-8f12-5fe1-ac05-e110056f3309
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 5
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 60.16
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 0de4d05b-0a54-11ec-b81f-005056a2bfc1
  Parent Event ID: 8e89c662-8f12-5fe1-ac05-e110056f3309
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 5
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 60.16
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: After 24 hour incubation

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 6a1445d8-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: 8e89c662-8f12-5fe1-ac05-e110056f3309
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 5
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 60.16
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 6b97658e-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: 8e89c662-8f12-5fe1-ac05-e110056f3309
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 5
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 60.16
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 5e26577a-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: d1ee951f-c608-5e2b-b07a-3d1fbab3a859
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 7
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 40.22
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: After 24 hour incubation

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 55a11f18-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: d1ee951f-c608-5e2b-b07a-3d1fbab3a859
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 7
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 40.22
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 60dcf46a-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: d1ee951f-c608-5e2b-b07a-3d1fbab3a859
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 7
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 40.22
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0
  

• Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  Event ID: 61d4debe-0741-11ec-b81f-005056a2bfc1
  Parent Event ID: d1ee951f-c608-5e2b-b07a-3d1fbab3a859
  Event date: 2021-08-28T12:04:10
  Station: P7 (NLEG25/NPAL16)
  Location: 81° 48' 9" N , 30° 53' 4" E
  Cruise number: 2021710
  Cruise name: 2021 Joint Cruise 2-2
  Bottle number: 7
  Sampletype: Primary production (14C-CO2)
  Sampling protocol: 7.22
  Gear type: CTD w/bottles
  Sample depth (m): 40.22
  Bottom depth (m): 3,055.62
  Person of Interest

  Name: Rolf Gradinger

  Email: rolf.gradinger@uit.no

  Institution: University of Tromsø
  Show more metadata

  Seasonality in primary production, Nitrogen sources for primary production and primary producers response to light intensity

  pi_name: Rolf Gradinger
  

  Abstract

  Several functional aspects of pelagic and simpagic primary producers were studied during the Nansen Legacy Q3 cruise. In open water stations (P1, P4, P5), water was sampled from the standard depths 10, 20, 40, 60, 90 and Chl a max. Water from these depths was spiked with radioactively labelled carbon in order to determine to the Carbon fixation rate (i.e. the Primary Production rate) of phototrophic organisms throughout the water column and latitudinal gradient. Additionally, water from 10m and Chla max was spiked with stable isotopes of Carbon (13C) and Nitrogen (15N) to determine the F-ratio (which fraction of the estimated primary production is new production). One incubation bottle was also treated to assess the nitrification activity of microbes. These incubations were deployed attached to the sediment trap moorings and exposed to in situ light and temperature conditions for 24 hours. In parallel, water from the Chla max was used to determine the photosynthetic response of the community to light intensity (P vs I curves). At the ice-covered stations (P6 and P7), the aforementioned water sampling and experimental work was carried as described, and additionally the bottom 3cm of 4 ice cores were sampled and pooled for similar in situ incubations: Under-ice Primary production, Nitrogen uptake and PvsI curve. Water from a meltpond was sampled, spiked and incubated in situ for Primary Production and Nitrogen uptake. A reciprocal transplant experiment was conducted with surface water from stations P1 and P6 and will be analyzed for abundance and community shifts due to changes in environmental factors.

  
  pi_email: rolf.gradinger@uit.no
  projectID: The Nansen Legacy (RCN # 276730)
  pi_address: Muninbakken 21, 9009 Tromsø
  recordedBy: Martí Amargant-Arumí
  pi_institution: UiT the Arctic University of Norway
  

  Created: 2021-10-12T11:45:30

  Modified: 2021-10-12T11:45:30

  History: 2021-10-12T11:45:30Z: Initial read in of the log files.

  Event remarks: Total carbon (t0) sample

  Source document: AeN_2021710_Primary_Production_Marti_04edits.xlsx

  statID: 466
  end_date: 2021-08-28 00:00:00
  end_time: 12:40:57.567000
  start_date: 2021-08-28 00:00:00
  vesselName: Kronprins Haakon
  intendedMethod: C14 incubations
  occurrenceRemarks: CTD w/bottles
  endDecimalLatitude: 81.80261895
  endDecimalLongitude: 30.88433095
  maximumDepthInMeters: 90
  minimumDepthInMeters: 0