Protocol as descibed in version 2 of the AeN protocols on p 108 (Primary production). The aim of my work on the first AeN cruise was to estimate the uptake rate of CO2 via different mechanisms and in different habitats in the Arctic. The studied habitats included water samples from the CTD rosette, sediment samples from the box corer, sea ice samples from the lowermost 1-2 cm of the ice cores, brine fluid from sack holes, melt pond water and algae aggregates. CO2 uptake was measured as photosynthetic uptake by microalgae and cyanobacteria, as nitrification by nitrifying bacteria and archaea, and as anapleurotic reactions (CO2 uptake into the TCA cycle) of heterotrophic microorganisms.
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