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Metadata Details
Metadata Details
Metadata Details
N-ICE2015 phytoplankton and ice algae taxonomy and abundance
, N-ICE2015, N-ICE2015
Collection:
SIOS
,
ADC
,
NSDN
Iso topic category:
biota
Show Abstract
The phytoplankton and ice-algae dataset contains counts of algae performed in an Imaging Flow Cytobot (IFCB) and in an inverted microscope. The counts were performed after the cruise using fixed samples collected from the sea ice and the water column. Quality METHODS: For algae samples ice cores were cut into 10-20 cm sections and melted overnight in cleaned opaque plastic containers with lids. 190-ml ice-algae samples were fixed with 25% glutaraldehyde and 20% hexamethylenetetramine-buffered formaldehyde at a final concentration of 0.1% and 1% (vol/vol), respectively, in 200-ml brown glass bottles. Phytoplankton were fixed in the same way, whereas more concentrated micro-plankton samples were fixed with 20% hexamine-buffered formaldehyde at a final concentration of 2%. All plankton samples were stored dark and cool. Algae were counted from Images collected with an Imaging Flow Cytobot (IFCB), as described by Laney and Sosik [2014]. Images were collected by triggering the IFCB to particle scattering. A Nitex screen with mesh size 130 μm prevented larger particles from clogging the flow-cell. All cells in 5 ml sample were counted. Algae were assigned to species/groups manually, using software written by Samuel R. Laney. Microscopy counts were performed after settling the cells in 50-mL Utermöhl chambers (HYDRO-BIOS©, Kiel, Germany) for 48 h. Algae were counted on parallel transects in an inverted Nikon Ti-U light microscope. For each sample a minimum of 50 cells of the dominant species were counted (i.e. maximum error of ± 28%). The “N-ICE2015_phytoplankton_icealgal_abundance_220415to050615” dataset contains microscopy counts (cells L-1) from young ice in a refrozen lead, and from the water column and the adjacent second-year/first-year ice (SYI/FYI) just before and during the time the young ice was studied (22.4.-5.6.2015). Samples have the following identifiers: Young ice: L_DATEsX_Y; L=lead, sX= coring sites 1-5; Y= B for bottom and T for top section of the ice core SYI/FYI: T_XDATE_YY; T=thick ice, X= M for main coring site (SYI), S for secondary coring site (FYI) (see papers), YY is the distance from the ice core bottom in cm (for the “start” i.e. the lower boundary of the ice core section) Water column: W_DATE_YY; W=water column, YY is sampling depth in m; in the case of several casts the same day (concerns 26.5. and 1.6.): d2605=Limnos sampling at dive tent, b0106=ship CTD cast 43 The dataset also includes the taxonomy sample ID (in column 2). In columns 3-7, some metadata (sampling date and depths) and a couple grouping variables are listed (e.g. for plotting). ‘Sampling day’ is a day count from 22.4.; ‘Habitat’: 1=young ice, 2=SYI/FYI, 3=water column. For more metadata and for biogeochemical data from the same ice cores see Assmy et al. 2017 (
https://doi.org/10.21334/npolar.2017.d3e93b31).
Following steps were done to clean the dataset: Species (categories) xx1, xx2 and xx1/xx2 were all summed together to category xx1/xx2. (example: Nitzschia frigida, Nitzschia neofrigida, Nitzschia frigida/neofrigida -> Nitzschia frigida/neofrigida) Diatom auxospores and hypnospores were summed together with other cells of the species. Species marked ‘cf. xx3’ are included in the category xx3. Completely unidentified taxa (except cysts) were not included in the analysis (concerns mainly the second-year/first-year ice). For the original data, contact the authors.
Show Keywords
GCMD Science Keyword
: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE
Metadata Information
Metadata identifier:
dc61cb24-c173-45c2-b8bc-e49afcd2aafe
Last metadata update:
2017-03-09T10:25:47Z
Last indexed:
2024-02-21T03:19:07Z
Alternate identifier:
Metadata status:
Active
Metadata update history
2017-03-09T10:25:47Z : Created : Made by transformation from DIF10 record
Related resources
Dataset Citation
Investigator
Name:
Lasse Mork Olsen, Philipp Assmy, Pedro Duarte, Samuel R. Laney, Mar Fernández-Méndez, Hanna Kauko, CJ Mundy, Jago Wallenschus, Anette Wold
Email:
lasse.mork.olsen@npolar.no
,
philipp.assmy@npolar.no
,
pedro.duarte@npolar.no
,
slaney@whoi.edu
,
mar.fernandez.mendez@npolar.no
,
hanna.kauko@npolar.no
,
anette.wold@npolar.no
Technical contact
Metadata Author
Data center
Short name:
Norwegian Polar Institute
Data center contact
Platform
Name:
Instrument
Name:
Temporal extent
Start date:
2015-01-20T12:00:00Z
End date:
2015-06-22T12:00:00Z
Geographic extent EPSG:4326
North:
83
South:
80
East:
27
West:
2
Observation location
Data access
File storage information
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